Roland Cy-5 Dual Trigger Cymbal Pad, 10In

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Load the reaction mixture (From "Run conjugation reaction") to the top of the SepHadex G-25 column.

The main application for cyanine dyes is in biological labeling. Nevertheless, there is a wide literature on both their synthesis and uses, and cyanines are common in some CD and DVD media. The amount of Cy5.5 required for reaction depends on the amount of protein to be labeled, and the optimal molar ratio of Cy5.5 to protein is about 10. Fuller, C. W. et al. The challenges of sequencing by synthesis. Nat. Biotechnol. 27, 1013–1023 (2009). Kretschy, N., Holik, A. K., Somoza, V., Stengele, K. P. & Somoza, M. M. Next-generation o-nitrobenzyl photolabile groups for light-directed chemistry and microarray synthesis. Angew. Chem. Int. Ed. 54, 8555–8559 (2015).CY-3 (长缨-3), which is a development of CY-2. Like CY-2, CY-3 can be launched from a variety of platforms, including aircraft, surface ships, submarines, land vehicles, and coastal shore batteries. However, despite the successful completion of the development and receiving state certification for service, only very limited number are in service with Chinese navy, mostly for long term evaluation purposes. CY-3 is facing stiff competition from another Chinese missile CJ-1, which is also under Chinese naval evaluation, and the final selection has not been decided yet.

by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic The pH value of protein solution shall be 8.5±0.5. If the pH is lower than 8.0, 1 M sodium bicarbonate shall be used for adjustment. requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make availableKvach, Maksim V.; Ustinov, Alexey V.; Stepanova, Irina A.; Malakhov, Andrei D.; Skorobogatyi, Mikhail V.; Shmanai, Vadim V.; Korshun, Vladimir A. (2008). "A Convenient Synthesis of Cyanine Dyes: Reagents for the Labeling of Biomolecules". European Journal of Organic Chemistry. 2008 (12): 2107–2117. doi: 10.1002/ejoc.200701190. ISSN 1099-0690.

Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. a b Ernst LA, Gupta RK, Mujumdar RB, Waggoner AS (Jan 1989). "Cyanine dye labeling reagents for sulfhydryl groups". Cytometry. 10 (1): 3–10. doi: 10.1002/cyto.990100103. PMID 2917472. Cyanines were first synthesized over a century ago. They were originally used, and still are, to increase the sensitivity range of photographic emulsions, i.e., to increase the range of wavelengths which will form an image on the film, making the film panchromatic. [4] Cyanines are also used in CD-R and DVD-R media. The ones used are mostly green or light blue colour, and are chemically unstable. For that reason, unstabilized cyanine discs are unsuitable for archival CD and DVD use. Recent cyanine discs contain stabilizers, typically a metal atom bonded to the cyanine molecule, [6] that slow the deterioration significantly. These discs are often rated with an archival life of 75 years or more. The other dyes used in CD-Rs are phthalocyanine and azo. Harvey, B. J., Perez, C. & Levitus, M. DNA sequence-dependent enhancement of Cy3 fluorescence. Photochem. Photobiol. Sci. 8, 1105–1110 (2009).CytoCy5S: The use of this product in an NTR gene reporter assay is the subject of US patent number 7,579,140 in the name of Cytiva.

Cy5 is sensitive to its electronic environment. Changes in the conformation of the protein it is attached to will produce either enhancement or quenching of the emission. The rate of this change can be measured to determine enzyme kinetic parameters. The dyes can be used for similar purposes in FRET experiments.Mujumdar, R. B., Ernst, L. A., Mujumdar, S. R., Lewis, C. J. & Waggoner, A. S. Cyanine dye labeling reagents—sulfoindocyanine Succinimidyl Esters. Bioconjugate Chem. 4, 105–111 (1993). Norman, D. G., Grainger, R. J., Uhrin, D. & Lilley, D. M. J. Location of cyanine-3 on double-stranded DNA: Importance for fluorescence resonance energy transfer studies. Biochemistry 39, 6317–6324 (2000). or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST Agbavwe, C. et al. Efficiency, error and yield in light-directed maskless synthesis of DNA microarrays. J. Nanobiotechnol. 9 (2011).