Operation Despicable Me Board Game

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Jain, M. et al. Improved data analysis for the MinION nanopore sequencer. Nat. Methods 12, 351–356 (2015). Pin the eye on the Minion: This is a classic game that is sure to please any minion fan. You will need a big image of a Minion, you could make your own by drawing a Minion of a huge piece of paper. Players take turns blindfolded, and try to pin the eye on the minion. The player who gets closest to the target wins the game. If you're looking for fun party game that is sure to please any minion fan, there are plenty of options to choose from. There are now minion-themed parties, costumes, and even video games. If you're looking to add a bit of minion- madness to your next party, there are plenty of options.

Gohl, D. M. et al. Systematic improvement of amplicon marker gene methods for increased accuracy in microbiome studies. Nat. Biotechnol. 34, 942–949 (2016). Suzuki, M. T. & Giovannoni, S. J. Bias caused by template annealing in the amplification of mixtures of 16S rRNA genes by PCR. Appl. Environ. Microbiol. 62, 625–630 (1996). Minion Twister: This is a great game for both children and adults. Players take turns contorting their bodies into different positions. The player who can do so without falling over wins the game. Mitsuhashi, S. et al. A portable system for rapid bacterial composition analysis using a nanopore-based sequencer and laptop computer. Sci. Rep. 7, 5657 (2017). According to the movies, minions are actually the result of an experiment gone wrong. Dr. Nefario, a mad scientist, was trying to create an army of valiant fighters. However, the results were not quite what he expected. Instead of brave warriors, he ended up with a race of small, timid creatures that were more interested in playing and eating bananas than fighting.Quail, M. A. et al. A tale of three next generation sequencing platforms: comparison of Ion Torrent, Pacific Biosciences and Illumina MiSeq sequencers. BMC Genom. 13, 341 (2012).

Lu, Q., Hu, H., Mo, J. & Shu, L. Enhanced amplification of bacterial and fungal DNA using a new type of DNA polymerase. Aust. Plant Pathol. 41, 661–663 (2012). The Minion Ring Toss:This game is great outdoor, you set up a Minion and take turns trying to toss rings onto the minion. The player who gets the most rings on the minion wins the game. Minion Uno: This is more of a quiet game for later in the party to wind the kids down a little. Each Players take turns playing cards, this is like the original Uno card game just Minion themed. they need to try to get rid of all of their cards. The first player to do so wins the game. Kai, S. et al. Rapid bacterial identification by direct PCR amplification of 16S rRNA genes using the MinION TM nanopore sequencer. FEBS Open Bio. 9, 548–557 (2019). Ferris, M. J., Muyzer, G. & Ward, D. M. Denaturing gradient gel electrophoresis profiles of 16S rRNA-defined populations inhabiting a hot spring microbial mat community. Appl. Environ. Microbiol. 62, 340–346 (1996).

Why MinION?

Guo, F., Ju, F., Cai, L. & Zhang, T. Taxonomic precision of different hypervariable regions of 16S rRNA gene and annotation methods for functional bacterial groups in biological wastewater treatment. PLoS ONE 8, e76185 (2013). Minion Simon Says: Players take turns following the instructions of the person who is "Simon." (Bob the Minion or Stuart the Minion "says") The player who follows the instructions correctly wins the game. Dave can be identified in this mini-movie. On his ID card, his photo shows that he's combed-haired, but he has buzz-cut hair at that time. Arai, S. et al. Assessment of pig saliva as a Streptococcus suis reservoir and potential source of infection on farms by use of a novel quantitative polymerase chain reaction assay. Am. J. Vet. Res. 79, 941–948 (2018). Meanwhile, the team is constantly working on new features, content & variety of improvements, which we hope will bring you only joy from playing the game in the future.

Minion Trivia: Players will need to answer questions about minions. The player with the most correct answers wins the game. This is great for the die hard Minion fans.

Minion Dance Party: Players simply dance to the music and have fun. The player who dances the best wins the game. Gru (the parents) will judge the best dancer and they will win a Minion themed prize. We recently received the Despicable Me 2 version of Monopoly and Operation Despicable Me 2 Silly Skill Game to review from Hasbro. The Operation game box includes the gameboard featuring Stuart the Patient and tweezers, 4 Minion Medics, 11 plastic Funatomy parts, 1 storage bag and game guide. Batteries are required and not included with the game. The 16S rRNA sequencing library was constructed according to the Illumina 16S Metagenomic Sequencing Library Preparation protocol (Illumina) targeting the V3 and V4 hypervariable regions of the 16S rRNA genes using primers 341F (5′-CCTACGGGNGGCWGCAG-3′) and 805R (5′-GACTACHVGGGTATCTAATCC-3′) 16. MightyAmp DNA Polymerase v. 2 (Takara Bio Inc.) was used for the PCRs. The initial PCR was performed using region-specific primers to ensure compatibility with the Illumina index and sequencing multiplex adapters. The amplified fragments were separated on 2% agarose gels, stained with Safelook Load-Green (Wako), and visualized on the FAS Nano Gel Document System (Nippon Genetics). The amount of purified DNA recovered was quantified using a Spectro/Fluorometer (DS-11FX+, DeNovix). An equimolar mixture of all PCR products was sent to a commercial company for 2 × 300 bp paired-end sequencing on the MiSeq platform using Illumina MiSeq v3 Reagent Kit (Fasmac, Kanagawa, Japan). Illumina sequencing data analysis D’Amore, R. et al. A comprehensive benchmarking study of protocols and sequencing platforms for 16S rRNA community profiling. BMC Genom. 17, 55 (2016).